Western blotting for the diagnosis of congenital toxoplasmosis.
نویسندگان
چکیده
Toxoplasmosis is a common congenital infection. It does not usually produce recognizable signs of infection at birth so most infected newborns are not detected by routine clinical examination and remain untreated. Infected children without clinical symptoms should nonetheless be identified and treated as early as possible. Serological diagnosis of congenital toxoplasmosis is quite difficult. The aim of this study was to evaluate the utility of Western blot for the diagnosis of congenital toxoplasmosis. We compared the immunological profiles of mothers and children to differentiate between passively transmitted maternal antibodies and antibodies synthesized by the infants in the first three months of life. The method enabled us to diagnose congenital toxoplasmosis in cases in which the infection had not been detected by classical serology techniques.
منابع مشابه
The investigation of congenital toxoplasmosis in a tertiary care hospital in Turkey.
OBJECTIVE To elucidate the prevalence of congenital toxoplasmosis in Turkey. METHODS This study was conducted in the Department of Parasitology, Faculty of Medicine, Ege University, Izmir, Turkey during the period of 2006 and 2007. Venous blood before delivery and cord blood during delivery were collected from 138 women, and we observed the presence of immunoglobulin (Ig)G and IgM antibodies ...
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Evaluation of a new protocol for retrospective diagnosis of congenital toxoplasmosis by use of Guthrie cards.
The aim of this study was to assess the diagnostic value of IgM Western blotting (WB), IgA enzyme immunoassay (EIA), and DNA amplification by real-time PCR on Guthrie cards to retrospectively establish the diagnosis of congenital toxoplasmosis (CT). To this purpose, Guthrie cards were collected from 18 infants born to mothers with primary Toxoplasma gondii infection during pregnancy. Moreover, ...
متن کاملPrenatal and Early Postnatal Diagnosis of Congenital Toxoplasmosis in a Setting With No Systematic Screening in Pregnancy.
To determine the risk of congenital toxoplasmosis (CT) and provide early (pre- or postnatal) identification of cases of CT in the absence of systematic screening in pregnancy.I n the presented cross-sectional study, serological criteria were used to date Toxoplasma gondii infection versus conception in 80 pregnant women with fetal abnormalities or referred to as suspected of acute infection, an...
متن کاملFirst Colombian Multicentric Newborn Screening for Congenital Toxoplasmosis
AIMS To determine the incidence of congenital toxoplasmosis in Colombian newborns from 19 hospital or maternal child health services from seven different cities of five natural geographic regions (Caribbean, Central, Andean, Amazonia and Eastern). MATERIALS AND METHODS We collected 15,333 samples from umbilical cord blood between the period of March 2009 to May 2010 in 19 different hospitals ...
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ورودعنوان ژورنال:
- The new microbiologica
دوره 34 1 شماره
صفحات -
تاریخ انتشار 2011